An unknown antibiotic, ophiocordin, C21H22N2O8, MW: 430, was isolated from submerged cultures of Cordyceps ophioglossoides, strain TÜ 276, grown in a glycerol soybean meal medium at 27°C. The antibiotic was extracted from acidified culture fluids with n-butanol and purified by subsequent column chromatography on DEAE-Sephadex and cellulose. Studies including nuclear magnetic resonance and mass spectrometry resulted in proposals of partial structures of the molecule. Inhibition by ophiocordin could be demonstrated for a small number of fungi belonging to different taxonomic groups. Bacteria were not inhibited. The antifungal effect was antagonized by ammonia and nitrate ions and by certain amino acids.
Metabolic products of microorganisms, 161st Communication. 160. Mitteilung: J. M. Müller, H. Fuhrer, J. Gruner, and W. Voser: Conocandin, ein fungistatisches Antibiotikum aus Hormococcus conorum (Sacc. et Roum.) Roback. Helv. chim. Acta 59, 2506–2514 (1976)