Various herbal medicines are thought to be useful in the management of cardiometabolic disease and its risk factors. Ganoderma lucidum (Curtis) P. Karst. (Ganodermataceae), also known as Lingzhi, has received considerable attention for various indications, including some related to the prevention and treatment of cardiovascular and metabolic disease by ameliorating major cardiovascular risk factors.
This review focuses on the major studies of the whole plant, plant extract, and specific active compounds isolated from G. lucidum in relation to the main risk factors for cardiometabolic disease.
References from major databases including PubMed, Web of Science, and Google Scholar were compiled. The search terms used were Ganoderma lucidum, Lingzhi, Reishi, cardiovascular, hypoglycaemic, diabetes, dyslipidaemia, antihypertensive, and anti-inflammatory.
A number of in vitro studies and in vivo animal models have found that G. lucidum possesses antioxidative, antihypertensive, hypoglycaemic, lipid-lowering, and anti-inflammatory properties, but the health benefits in clinical trials are inconsistent. Among these potential health benefits, the most compelling evidence thus far is its hypoglycaemic effects in patients with type 2 diabetes or hyperglycaemia.
The inconsistent evidence about the potential health benefits of G. lucidum is possibly because of the use of different Ganoderma formulations and different study populations. Further large controlled clinical studies are therefore needed to clarify the potential benefits of G. lucidum preparations standardised by known active components in the prevention and treatment of cardiometabolic disease.
Cardiovascular disease (CVD) is highly prevalent, with ischaemic heart disease and stroke being the two leading causes of mortality throughout the world (World Health Organization 2021). Metabolic syndrome is characterised by a cluster of conditions including insulin resistance, central obesity, hypertension, dyslipidaemia, and low-grade chronic inflammation (Eckel et al. 2005). Several drug treatments for CVD have been derived from plant sources, such as digoxin and reserpine. Herbal medicines are now becoming more popular, representing a potentially cost-effective class of substances for combating CVD if safe and effective therapies can be identified. The common herbal medicines used in the West include Asian ginseng, astragalus, flaxseed oil, garlic, ginkgo, grape seeds, green tea, hawthorn, milk thistle, and soy (Liperoti et al. 2017). Herbal formulae are widely used in the clinic in China for hypertension, dyslipidaemia, coronary heart disease, and heart failure (Liu and Huang 2016).
Ganoderma (Ganodermataceae) is a kind of woody mushroom that can be found all over the world. Individual members of the species are identified according to different characteristics, such as shape and colour (red, black, blue/green, white, yellow, and purple) of the fruiting bodies, host specificity, and geographical origin (Upton 2000; Wachtel-Galor et al. 2011). Ganoderma lucidum (Curtis) P. Karst. (Curtis 1781), known as Lingzhi in China and Reishi in Japan, has been used in traditional Chinese medicine (TCM) for over 2000 years for a broad range of indications including improving general health, well-being, and longevity (Bishop et al. 2015; Klupp et al. 2015).
A variety of commercial products from G. lucidum, such as powders, dietary supplements, and tea (Wachtel-Galor et al. 2011), are available. They have been shown to possess a range of activities against CVD, including effects on lipids, blood pressure, obesity, diabetes, and antioxidant and radical scavenging properties (Liu and Tie 2019; Meng and Yang 2019; Winska et al. 2019). However, scientific evidence supporting the beneficial medical properties of G. lucidum is still inconclusive (Hapuarachchi et al. 2016). Many of the commercial products from G. lucidum may not have undergone effective standardisation, so it is difficult to compare results from different studies with different products. Many different herbal supplements or nutraceutical commercial products bearing the names Lingzhi, Reishi, or Ganoderma, etc., contain extracts from various parts of G. lucidum, often in combination with other herbal components. Ganopoly™ (Encore Health), which is a product containing water-soluble G. lucidum polysaccharides, has been used in some animal and clinical studies.
In this review, the major studies of the whole plant, plant extract, and specific active compounds isolated from G. lucidum in relation to the main risk factors for CVD with particular emphasis on the more recent studies, are summarised. Electronic literature searches were performed using PubMed, Web of Science, and Google Scholar (published from 1961 to 2021). The search terms used were Ganoderma lucidum, Lingzhi, Reishi, cardiovascular, hypoglycaemic, diabetes, dyslipidaemia, antihypertensive, and anti-inflammatory. A total of 4224 articles were identified. The bibliographies of all relevant articles thus located were also scanned for further relevant references. S.W.C and B.T. extracted all articles independently based on the relevance, quality, and strength of the studies; only a shortlist of 115 studies or representative findings are discussed below.
Active constituents of G. lucidum
G. lucidum is thought to have numerous different biologically active constituents, the main ones being various triterpenes, polysaccharides, and proteins (Ahmad 2018; Ahmad et al. 2013). The pharmacologically active compounds are present in different amounts in various parts of the mushroom such as the fruiting bodies, mycelium and spores.
Terpenes are a large and diverse group of naturally occurring compounds derived from the branched C5 carbon skeleton of isoprene. Triterpenes are a subclass of terpenes and are derived from squalene, a C30 hydrocarbon (Abdullah et al. 2012). They can be classified based on the number of cyclic structures making up the compounds. Up to now, more than 150 triterpenes have been identified from the spores, fruiting bodies, and mycelia of G. lucidum (Xia et al. 2014; Baby et al. 2015). The methods of extraction of triterpenes usually involve methanol, ethanol, chloroform, ether, acetone, or a mixture of these solvents. The extracts can be further purified by various separation methods such as normal and reverse-phase high-performance liquid chromatography (HPLC) (Chen et al. 1999). The majority of triterpenes identified are ganoderic acids and lucidenic acids; other important triterpenes include ganodermic acids, ganoderals, and ganoderiols (Wachtel-Galor et al. 2011). The strong bitterness of G. lucidum originates from the triterpenoid compounds and the bitterness depends on the strain, cultivation conditions and manufacturing processes (Seo et al. 2009). Triterpenoids have been reported to exhibit various biological activities including anti-hypertensive, lipid-lowering, anti-acetylcholinesterase, antioxidant, and anticancer activities, etc. (Abdullah et al. 2012; Chen et al. 2017).
Polysaccharides and peptidoglycans
G. lucidum polysaccharides are macromolecules with a molecular mass of above 500 kDa. Many different polysaccharides, including (1→3), (1→6)-α/β-glucans, α-d-glucans, α-d-mannans, and polysaccharide-protein complexes, have been identified from the spores, fruiting bodies and mycelia of G. lucidum. These compounds are reported to have immunomodulatory and anticancer activities (Xu et al. 2011; Kao et al. 2013). Glucose, together with xylose, mannose, galactose, and fucose in different conformations, forms the major component of the polysaccharide molecules. Polysaccharides are the major component by weight among all constituents in the spores. Several of the mushroom polysaccharide compounds have proceeded through Phase I, II, and III clinical trials and have been used in some Asian countries to treat various cancers and other diseases (Wasser 2010). The contents of polysaccharides differ among commercial Lingzhi products (Wachtel-Galor et al. 2011). A polysaccharide-based product extracted from the spores of G. lucidum originally named ‘Ji 731 Injection’ was used since 1973 in China for treating myopathy (Zeng et al. 2018). The drug was renamed ‘Ji Sheng Injection’ in 1985 and subsequently ‘Polysaccharidum of G. lucidum Karst Injection’ (Lin Bao Duo Tang Zhu She Ye) and is still used for intramuscular injection for various types of immune-mediated muscle diseases. Various bioactive peptidoglycans possessing antiviral (Li et al. 2005) and immunomodulating activities (Zhang et al. 2019), such as ganoderans A, B, and C, have also been isolated from G. lucidum.
Several bioactive proteins from G. lucidum have been reported. One of these is a polypeptide called Lingzhi-8 (LZ-8) which consists of 110 amino acids with a molecular mass of 12 kDa. It has an immunoglobulin-like structure and was the first immunomodulatory protein isolated from the mushroom in 1989 (Hsu and Cheng 2018). Another protein from the fruiting bodies of G. lucidum is ganodermin, which has a molecular mass of 15 kDa and has antifungal activity.
Health benefits of G. lucidum
Free radicals are unstable and highly reactive chemical entities which contain one or more unpaired electrons and can be uncharged or charged. Free radicals are beneficial to the cell signalling and immune system, as well as maintenance of normal body functioning. However, excessive formation and/or insufficient removal of reactive oxygen species (ROS) and reactive nitrogen species (RNS), known as ‘oxidative stress’, may modulate the blood vessel wall, creating an environment that facilitates the progression of atherosclerosis, and leading to various illnesses, such as heart disease, diabetes and cancer (Johansen et al. 2005; Ullah et al. 2016).
In vitro studies demonstrated that several constituents of G. lucidum, in particular triterpenoids and polysaccharides, exhibit antioxidant activity, reducing power, scavenging and chelating abilities (Mau et al. 2002; Saltarelli et al. 2009; Wu and Wang 2009; Liu et al. 2010; Sarmadi and Ismail 2010; Kozarski et al. 2011; Ferreira et al. 2015; Krishna et al. 2016). In contrast, polysaccharide extracts of G. lucidum have superoxide and hydroxyl radical scavenging activities but do not have antioxidative activity as measured by detecting malondialdehyde (MDA) contents of liver microsomes (Liu et al. 1997). It has been demonstrated that the phenolic compounds from the fresh fruiting bodies of G. lucidum exhibit strong 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity but low superoxide dismutase (SOD) activity. The study also showed that DPPH radical scavenging activity and SOD activity were positively correlated with phenolic compounds including caffeic acid, catechin, ferulic acid, gallic acid, myricetin, naringin, pyrogallol, protocatechuic acid, homogentisic acid, and quercetin, as well as total phenolic compounds (Kim et al. 2008). A study comparing the antioxidant activities of four of the most widely known mushrooms, including G. lucidum, demonstrated that polysaccharide extracts exhibited a strong correlation between the reducing power and the total amount of phenols and α-glucans, while a correlation between the reducing power and the amount of total polysaccharides and proteins was not found (Kozarski et al. 2012).
In vivo studies have shown that G. lucidum increases the activity of the antioxidant enzymes SOD and catalase (CAT), which are involved in removing harmful ROS (Cherian et al. 2009; Yurkiv et al. 2015; Vitak et al. 2017; Rahman et al. 2018). In an ischaemia and reperfusion isolated perfused rat heart model, administration of G. lucidum extract (400 mg/kg for 15 days) exhibited antioxidant properties and the author concluded that the cardioprotective properties of G. lucidum extract are related to its antioxidant effects (Lasukova et al. 2015). A study in rats showed that G. lucidum ethanol extract (250 mg/kg body weight) ameliorated the cardiotoxicity of adriamycin by reducing the increase in lipid peroxidation and reversing the decrease in the antioxidant enzymes, glutathione peroxidase (GPx), glutathione-S-transferase (GST), SOD and CAT in the heart tissue (Rajasekaran and Kalaimagal 2012). The cardioprotective effect of G. lucidum may be attributed to the antioxidant chemicals triterpenes and polysaccharides (Wachtel-Galor et al. 2004b). In a carotid-artery-ligation mouse model, daily oral G. lucidum (300 mg/kg/day) prevented neointimal thickening 2 weeks after ligation. Furthermore, subcutaneous injections of ganoderma triterpenoid (GT) crude extract (300 mg/kg/day) abolished ligation-induced neointima formation. The authors concluded that GTs prevent atherogenesis by eliminating disturbed flow-induced oxidative stress through inhibiting the induction of a series of atherogenic factors, as well as inflammation (Hsu et al. 2018).
A short-term supplementation study over 10 days in healthy subjects showed an improvement in antioxidant status (Wachtel-Galor et al. 2004a), but a longer double-blind, placebo-controlled, cross-over intervention study over 4 weeks with a commercially available encapsulated Lingzhi preparation (1.44 g Lingzhi/day; equivalent to 13.2 g fresh mushroom/day) showed no significant effects in a range of biomarkers for antioxidant status, cardiovascular risk, DNA damage, immune status, and inflammation (Wachtel-Galor et al. 2004b). A placebo-controlled cross-over study in 42 healthy subjects examined the antioxidation and hepatoprotective efficacy of triterpenoids and polysaccharide-enriched G. lucidum, which was taken as a 225 mg capsule containing 7% triterpenoid-ganoderic acid (A, B, C, C5, C6, D, E and G), 6% polysaccharide peptides with a few essential amino acids and trace elements, once daily for 6 consecutive months (Chiu et al. 2017). The treatment showed an improvement in total antioxidant capacity, total thiols and glutathione content in plasma, significantly enhanced activities of antioxidant enzymes (SOD, CAT, GPx and glucose-6-phosphate dehydrogenase), and reduced the levels of thiobarbituric acid reactive substances, 8-hydroxy-deoxy-guanosine and hepatic marker enzymes, glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase. Mild fatty liver detected by abdominal ultrasonic examination was reversed to normal with G. lucidum treatment.
Hyperglycaemia may increase the susceptibility to lipid peroxidation and modulate glucose metabolism in the body, which ultimately contributes to the increased incidence of atherosclerosis or further accelerates its progression (Giugliano et al. 1996; Poznyak et al. 2020). Insulin treatment is essential for people with type 1 diabetes. In type 2 diabetes mellitus (T2DM), lifestyle modification is recommended. If lifestyle modification is not sufficient in achieving glycemic control, patients should be treated initially with metformin (American Diabetes Association 2020). Metformin belongs to the biguanide class of drugs, which originate from the plant goat’s rue or French lilac (Galega officinalis, Linnaeus, [Fabaceae]) (Witters 2001). Recently, the glucagon-like peptide 1 (GLP-1) receptor agonists and sodium-glucose cotransporter 2 (SGLT2) inhibitors, which were developed from phlorizin, a natural compound isolated from the bark of apple roots (Tomlinson et al. 2017), have been considered suitable for first-line treatment in some patients with T2DM who have concomitant cardiac or renal disease, in order to improve cardiovascular outcome benefits (Davies et al. 2018).
The hypoglycaemic effects of various extracts from G. lucidum have been studied in different animal models of diabetes and in in vitro experiments to identify mechanisms (Ma et al. 2015; Wang et al. 2016; Winska et al. 2019). The main in vitro, animal and clinical studies investigating the hypoglycaemic effects of G. lucidum are summarised in Tables 1–3, respectively.
|Zhang et al. 2003||Alloxan-induced pancreatic islet damage||Gl-PS polysaccharides from the fruiting body of G. lucidum||Gl-PS showed a protective effect|
|Fatmawati et al. 2009||Human aldose reductase activity||Methanol extracts of 17 medicinal and edible mushrooms||G. lucidum showed the highest aldose reductase inhibitory activity|
|Fatmawati et al. 2010||Human aldose reductase activity||Ganoderic acid Df isolated from the fruiting body of G. lucidum||Ganoderic acid Df showed potent human aldose reductase inhibitory activity|
|Fatmawati et al. 2011a||Human α-glucosidase activity||Chloroform extract of the fruiting body of G. lucidum||Ganoderol B identified as an active α-glucosidase inhibitor|
|Pan et al. 2015||PTP1B activity||FYGL proteoglycan isolated from G. lucidum||Competitive inhibitor of PTP1B|
|Yang et al. 2018a||Liver tissues of ob/ob mice and HepG2 cells||FYGL proteoglycan isolated from G. lucidum||Inhibited PTP1B overexpression, improved IRS1 phosphorylation, activated PI3K/Akt cascades, increased phosphorylation of GSK3β, enhanced insulin-stimulated glycogen synthesis|
|Yang et al. 2018b||Rat myoblast L6 cells||FYGL proteoglycan isolated from G. lucidum||Increased insulin-stimulated glucose uptake, inhibited PTP1B expression, increased IRS1 phosphorylation, activated PI3K/Akt, increased phosphorylation of AMPK and up-regulated expression of GLUT4|
|Hikino et al. 1985||Normal and alloxan-induced hyperglycaemic mice||Water extracts (104 mg/kg crude drug equivalent, i.p.) of the fruiting bodies of G. lucidum for 7 or 27 h||Reduced plasma glucose and 2 glycans, ganoderans A and B, with hypoglycaemic action isolated|
|Hikino et al. 1989||Normal and glucose-loaded mice||Ganoderan B||Increased insulin and altered enzyme activities|
|Kino et al. 1990||Autoimmune diabetes model in non-obese mice||Ling Zhi-8 immunomodulatory protein (10.3 − 12.6 mg/kg twice weekly) from 4 weeks of age, followed up to 42 weeks of age||Prevented development of autoimmune diabetes by immunosuppressive mechanism|
|Zhang et al. 2003||Alloxan-induced diabetic mice||Pre-treatment with intragastric Gl-PS (50 − 200 mg/kg) for 10 days||Gl-PS partly protected beta cells from necrosis|
|Zhang & Lin 2004||Normal fasted mice||Gl-PS (25 − 100 mg/kg) given by single intraperitoneal injections||Reduced serum glucose and increased insulin levels|
|He et al. 2006||Streptozotocin-induced diabetic mice||Gl-PS (125 and 250 mg/kg) given for 8 weeks||Reduced serum glucose, increased insulin levels and delayed progression of diabetic renal disease|
|Seto et al. 2009||Genetically obese/diabetic (+db/+db) and lean (+db/+m) mice||Water extract of G. lucidum (0.003, 0.03 and 0.3 g/kg) for 4 weeks, oral gavage||Extract reduced serum glucose and liver|
|Li et al. 2011||Streptozotocin-induced diabetic mice||Gl-PS at low (50 mg/kg) and high (150 mg/kg) dose for 28 days||Reduced serum glucose, increased insulin levels and improvements in blood lipids|
|Teng et al. 2011||Streptozotocin-induced diabetic mice||FYGL proteoglycan from G. lucidum (50 and 150 mg/kg, oral dose) for up to 4 weeks||Reduced plasma glucose with effect comparable with metformin|
|Teng et al. 2012||Streptozotocin-induced diabetic rats||FYGL proteoglycan from G. lucidum (40 and 120 mg/kg, oral dose) for 30 days||Reduced plasma glucose, increased insulin and inhibited PTP1B|
|Zheng et al. 2012||Streptozotocin-induced diabetic rats||Low-molecular-weight Gl-PS (200 mg/kg) orally for 8 weeks||Reduced serum glucose appeared related to protection of pancreatic β-cells|
|Xiao et al. 2012||Streptozotocin-induced diabetic mice||Polysaccharides from G. lucidum (50 or 100 mg/kg/day) given for 7 days||Reduced fasting serum glucose and insulin levels|
|Pan et al. 2013||Obese/diabetic (+db/+db) mice||FYGL proteoglycan from G. lucidum (75, 250, or 450 mg/kg) for 8 weeks||Reduced HbA1c, increased insulin and C-peptide levels, increased glucokinase and lowered PEPCK activities|
|Sarker 2015||Rats with alloxan- or corticosteroid-induced diabetes||A petroleum ether extract and a methanol extract of G. lucidum (200, 400, 600 and 800 mg/kg/day) for 7 days||Reduced fasting and postprandial plasma glucose and HbA1c, increased plasma insulin levels and improved lipid profile|
|Xiao et al. 2017||Streptozotocin-induced diabetic mice||F31 polysaccharide from G. lucidum (50 mg/kg/day)||Decreased fasting serum glucose, fasting serum insulin and liver glucose regulatory enzymes|
|Ratnaningtyas et al. 2018||Alloxan-induced diabetic rats||Ethanol extract of G. lucidum powdered fruiting bodies (250, 500 and 1000 mg/kg) for 14 days||Dose-dependent reduction in blood glucose, reduction in HbA1c, and increase in insulin|
|Bach et al. 2018||Streptozotocin-induced diabetic rats||Hydroethanolic extract of G. lucidum (1 mL/kg/day) for 30 days||Reduced plasma glucose and lipid levels|
|Gao et al. 2004b||62 patients with T2DM||Multi-centered randomised controlled trial of GanopolyTM 1800 mg 3 times daily versus placebo for 12 weeks||Reduced HbA1c and fasting and postprandial plasma glucose levels with GanopolyTM|
|Wang et al. 2008||46 patients with T2DM||Randomised, double-blind, placebo-controlled dry extract of G. lucidum 3000 mg or placebo for 12 weeks||No changes in fasting glucose or HbA1c but the plasma glucose area under the curve during a meal tolerance test was reduced more with G. lucidum extract|
|Chu et al. 2012||23 subjects with borderline elevations of blood pressure and/or cholesterol||Randomised, double-blind, cross-over study with a Lingzhi product 1.44 g daily or placebo for 12 weeks||No significant effect on HbA1c, fasting plasma glucose, blood pressure or lipids. Plasma insulin and HOMA-IR reduced with Lingzhi compared to placebo|
|Klupp et al. 2016||84 patients with T2DM and metabolic syndrome||Randomised controlled trial of G. lucidum 3 g/day or G. lucidum plus Cordyceps sinensis capsules, versus placebo for 16 weeks||No significant effect on HbA1c, fasting plasma glucose, blood pressure or lipids|
Hypoglycaemic activity of triterpenoids
A series of in vitro studies by Fatmawati and colleagues have identified that methanol extract from the fruiting bodies of G. lucidum has a strong inhibitory effect on human aldose reductase activity. Ganoderic acid Df (Figure 1), a lanostane-type triterpenoid, exhibited potent aldose reductase inhibitory activity with an IC50 value of 22.8 µM (Fatmawati et al. 2009, 2010). Fatmawati et al. (2011a) subsequently demonstrated that ganoderol B (Figure 2), which was isolated from a chloroform extract of G. lucidum, was effective in inhibiting α-glucosidase activity with an IC50 value of 119.8 µM and the inhibitory effect was stronger than that of acarbose, which is commonly used as a medication to inhibit α-glucosidase in patients with T2DM. Structure-activity studies were performed to identify the structural requirements of lanostane-type triterpenoids from G. lucidum, which were necessary to increase α-glucosidase inhibitory activity (Fatmawati et al. 2013).
Hypoglycaemic activity of Ganoderma polysaccharides Hypoglycaemic effects of polysaccharides from G. lucidum (Gl-PS) have been demonstrated in several in vitro and in vivo studies. Gl-PS showed a protective effect against alloxan-induced damage to pancreatic islets in vitro. Pre-treatment with intragastric Gl-PS (50-200 mg/kg) for 10 days produced hypoglycaemic effects via its scavenging ability to protect the pancreatic β-cells from alloxan-induced necrosis (Zhang et al. 2003). Gl-PS (25-100 mg/kg) given by single intraperitoneal injections to normal fasted mice reduced serum glucose levels after 3 and 6 h in a dose-dependent manner and increased insulin levels from 1 h after administration via enhancing Ca2+ influx into pancreatic β cells (Zhang and Lin 2004). Furthermore, administration of Gl-PS produced hypoglycaemic effects and an improvement in lipid profile in streptozotocin-induced diabetic mice (He et al. 2006; Li et al. 2011; Zheng et al. 2012). It has been suggested that the hypoglycaemic effect is mainly through preventing apoptosis of pancreatic β-cells and enhancing β-cells regeneration (Zheng et al. 2012), and a modulation of serum insulin and hepatic mRNA levels of several key enzymes involved in gluconeogenesis and/or glycogenolysis, including GP, fructose-1,6-bisphosphatase (FBPase), phosphoenolpyruvate carboxykinase (PEPCK), and G6Pase (Xiao et al. 2012). Xiao et al. (2017) isolated F31, a β-heteropolysaccharide with a weight-average molecular weight of 15.9 kDa, from Gl-PS. The mechanism of action of Gl-PS F31 may be associated with down-regulation of the hepatic glucose regulated enzyme mRNA levels via AMPK activation, improvement of insulin resistance, and reduction of epididymal fat/body weight ratio (Xiao et al. 2017). An integrative analysis of transcriptomics and proteomics data from the liver from F31-treated diabetic db/db mice found that genes in the glycolysis and gluconeogenesis pathways, insulin pathway, and lipid metabolism pathways showed significantly different expression compared to the untreated mice and that microRNAs probably participated in the regulation of the genes involved in glucose metabolism (Xiao et al. 2018).
Hypoglycaemic activity of Ganoderma extracts Some other studies used extracts of G. lucidum in which the active constituents were not clearly identified. A water-extract of G. lucidum given to lean (+db/+m) and genetically obese/diabetic (+db/+db) mice lowered the serum glucose level in + db/+db mice after one week of treatment and in + db/+m mice after 4 weeks, through the down-regulation of the hepatic PEPCK gene expression (Seto et al. 2009). A study in alloxan-and steroid-induced diabetic rats showed that a petroleum ether extract and a methanol extract of G. lucidum given orally at 200, 400, 600 and 800 mg/kg/day for 7 days reduced plasma glucose levels, increased insulin sensitivity, and decreased lipid levels, and the suspected bioactive chemicals were polysaccharides available in the extracts (Sarker 2015). A hypoglycaemic effect was also observed following administration of an alcoholic extract of G. lucidum (250, 500, and 1000 mg/kg) given for 14 days in alloxan-induced diabetic rats (Ratnaningtyas et al. 2018). Another recent study in streptozotocin-induced diabetic rats showed that a hydroethanolic extract of G. lucidum containing β-glucan, proteins, and phenols, reduced plasma glucose and lipid levels through preservation of pancreatic islets (Bach et al. 2018).
Hypoglycaemic activity of Ganoderma proteins Ling Zhi-8 (LZ-8), an immunomodulatory protein isolated from the mycelial extract of G. lucidum, prevented the development of autoimmune diabetes by reducing antigen-induced antibody formation in non-obese diabetic mice (Kino et al. 1990). In a model of transplanted allogeneic pancreatic rat islets, LZ-8 delayed the rejection process of allografted islets (van der Hem et al. 1995).
Evidence from clinical studies Clinical studies of the hypoglycaemic/antidiabetic effects of G. lucidum products are very limited. In a placebo-controlled study in 62 patients with T2DM, administration of Ganopoly™ at 1800 mg three times daily for 12 weeks reduced fasting and postprandial plasma glucose levels, as well as HbA1c (Gao et al. 2004b). Administration of a dry extract of G. lucidum (3 g) in addition to regular oral hypoglycaemic agents for 12 weeks did not affect fasting glucose or HbA1c; however, the plasma glucose area under the curve during a meal tolerance test was reduced more significantly in patients taking G. lucidum (Wang et al. 2008). A randomised, double-blind, placebo-controlled, cross-over study with placebo-controlled run-in and cross-over periods of a Lingzhi product at a dose of 1.44 g daily for 12 weeks was performed in subjects with borderline elevations of blood pressure and/or cholesterol. There were reductions in plasma insulin and homeostasis model assessment-insulin resistance with Lingzhi compared to placebo. The subjects in this study had normal plasma glucose levels and it was speculated that the effects on insulin and insulin resistance would be greater in subjects with impaired glucose tolerance or T2DM (Chu et al. 2012). However, in a more recent study in 84 patients with T2DM and metabolic syndrome, administration of G. lucidum alone or combined with Cordyceps sinensis [now called Ophiocordyceps sinensis (Berk.) Sacc. (Ophiocordycipitaceae)], over 16 weeks, did not show any improvement in hyperglycaemia and cardiovascular risk factors (Klupp et al. 2016). It is noteworthy that different extracts of G. lucidum will have different components, therefore it may not be appropriate to compare the results from different studies.
Effects on dyslipidaemia
Dyslipidaemia which is characterised by decreased levels of HDL-C and accompanied with increased levels of TG, apo B, and small dense LDL particles, is an important modifiable risk factor for the development of atherosclerosis and CVD. Guidelines for the treatment of lipid disorders recommend initiating treatment with the 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductase inhibitors or statins (Grundy et al. 2019; Mach et al. 2020). Statins have their origin in products isolated from fungi (Endo 2004).
In vitro studies showed that polysaccharides and oxygenated triterpenoids from G. lucidum have a very broad spectrum of biological activities and pharmacological effects. Some types of ganoderic acid might reduce cholesterol by inhibiting HMG-CoA reductase, like the statin drugs (Shiao 2003). Compounds isolated from fruiting bodies of G. lucidum including ganolucidic acid eta, ganoderenic acid K, and the farnesyl hydroquinones (ganomycin J and ganomycin B), showed strong inhibitory activity against HMG-CoA reductase (Figure 3) (Chen et al. 2017).
The cholesterol-lowering properties of G. lucidum have been demonstrated in a series of in vitro and ex vivo studies, and in hamsters and minipigs (Berger et al. 2004). The organic fractions containing oxygenated lanosterol derivatives inhibited cholesterol synthesis in T9A4 hepatocytes. The investigators found that both 2.5 and 5% dried G. lucidum reduced hepatic microsomal ex-vivo HMG-CoA reductase activity. In hamsters, administration of 5.0% dried G. lucidum decreased TC and HDL-C but not LDL-C, whereas in minipigs, 2.5% dried G. lucidum reduced all these parameters.
The improvements in the lipid profile in some diabetic animal models and in patients with T2DM treated with G. lucidum products may be related to the improvement in glycemic control, rather than a direct effect on lipid metabolism as hyperglycaemia is often associated with elevated TG and reduced HDL-C (Taskinen and Borén 2015). In a randomised, double-blind, cross-over study in 26 patients with borderline elevations of blood pressure and/or cholesterol, administration of Lingzhi (1.44 g extract/d) for 12 weeks produced a non-significant trend for reduction in TG and increase in HDL-C (Chu et al. 2012). Those changes could have been related to improvements in insulin resistance as these lipid abnormalities, hypertension, central obesity and insulin resistance cluster together in the metabolic syndrome.
The most recent guidelines for the management of hypertension recommend initiating antihypertensive drug therapy in most patients with a combination of two different drugs from the classes of thiazide diuretics, calcium channel blockers, angiotensin converting enzyme (ACE) inhibitors, or angiotensin receptor blocker (ARBs) (Whelton et al. 2018; Williams et al. 2018).
Triterpenes and G. lucidum proteins have been demonstrated to possess potent ACE-inhibitory properties in vitro (Abdullah et al. 2012; Mohamad Ansor et al. 2013). Mohamad Ansor et al. (2013) reported that the protein fractions from the mycelia of G. lucidum contain highly potent anti-ACE proteins with IC50 values below 200 μg/mL. Furthermore, three small peptides with ACE-inhibitory activity, including Gln-Leu-Val-Pro (QLVP), Gln-Asp-Val-Leu (QDVL), and Gln-Leu-Asp-Leu (QLDL), were recently isolated from G. lucidum mycelia (Wu et al. 2019). Notably, QLVP worked in a mixed-type manner against ACE and has an IC50 value of 127.9 µmol/L.
A transverse aortic constriction (TAC) mouse model of pressure overload-induced cardiomyopathy and heart failure revealed that administration of oral Ganoderma spore oil every other day for 14 days normalised ejection fraction, corrected the fractional shortening and reduced left ventricular hypertrophy. The cardioprotective effect is associated with reduced expression of circular RNA circ-Foxo3, which plays a role in the pathogenesis of heart failure (Xie et al. 2016).
An early uncontrolled trial in Japanese showed that supplementation with G. lucidum extract (240 mg daily) for 6 months reduced blood pressure in hypertensive patients but not borderline hypertensive or normotensive patients (Kanmatsuse et al. 1985). In a double-blind, randomised, placebo-controlled study in 160 patients with confirmed coronary heart disease (CHD), treatment with G. lucidum polysaccharides (Ganopoly™) for 12 weeks improved the symptoms of CHD and reduced average blood pressure from 142.5/96.4 mmHg to 135.1/92.8 mmHg, whereas there was no significant blood pressure reduction in the control group (Gao et al. 2004a). Serum TC also decreased significantly with Ganopoly™ therapy, but not in the control group.